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FISABIO-Public Health Sequencing and Bioinformatics Service

Pricing

FISABIO’S PLATFORM AND SERVICES UNIT

Illumina Sequencing

 

AMPLICONS: library + sequencing
Type rate per sample
A B C
Amplicon libraries(16S, 18S, ITS, o custom) and sequencing in illumina MiSeq, 2x300pb run. Minimum 60.000 reads per sample 56€ 58€ 60€

 

Microbial genomes MiSeq 2x300pb Type rate per sample
A B C
Microbial genomic DNA library preparation and sequencing in illumina MiSeq, 2x300pb run (COVERAGE 100X) 214€ 218€ 230€
Microbial genomic DNA library preparation and sequencing in illumina MiSeq, 2x300pb run (COVERAGE 50X) 138€ 146€ 159€
Microbial genomic DNA library preparation and sequencing in illumina MiSeq, 2x300pb run (COVERAGE 20X) 103€ 105€ 115€

 

Microbial genomes NextSeq 2x150pb Type rate per sample
A B C
Microbial genomic DNA library preparation and sequencing in illumina NextSeq, 2x150pb run (COVERAGE 100X) 137€ 139€ 151€
Microbial genomic DNA library preparation and sequencing in illumina NextSeq, 2x150pb run (COVERAGE 50X) 106€ 108€ 118€
Microbial genomic DNA library preparation and sequencing in illumina NextSeq, 2x150pb run (COVERAGE 20X) 100€ 103€ 110€

 

Metagenomes NextSeq 2x150pb Type rate per sample
A B C
Metagenomes (50 Millions reads per sample) 886€ 900€ 982€
Metagenomes (20 Millions reads per sample) 500€ 508€ 554€
Metagenomes (10 Millions reads per sample) 292€ 298€ 325€

 

Viral genomes MiSeq 2x300pb Type rate per sample
A B C
Viral genomes (COBERTURA 1000X) 85€ 87€ 94€
Viral genomes (COBERTURA 10.000X) 214€ 218€ 238€

 

Microbial RNA-SEQ Nextseq 1x150pb Type rate per sample
A B C
RNA-seq / metatranscriptomes (50 million reads per sample) 768€ 781€ 851€
RNA-seq / metatranscriptomes (20 million reads per sample) 393€ 401€ 437€
RNA-seq / metatranscriptomes (10 million reads per sample) 281€ 285€ 312€

 

 

Other services Unique price (€)
Ribosomal RNA depletion and DNAse treatment 70
DNA extraction 20
RNA extraction 25
DNA quantification 1
DNA fragment size measurement 25
Amplicon libraries sequencing 22
IIndexing and amplicon libraries sequencing 50

 

 

KIND OF PROCESS: Sequencing runs Type rate per sample OUTPUT (Gb / Single read)
Unique price
MiSeq® Reagent Kit v3 (600 cycles) 2100,00 15Gbp / 25 MLN
MiSeq Reagent Kit v2 (500cycles) 1700,00 8,5Gbp /15 MLN
MiSeq Reagent Kit v3 (150-cycles) 1100,00 3,8Gbp /25 MLN
MiSeq Reagent Kit v2 (300 cycles) 1600,00 5 Gbp /15 MLN
MiSeq Reagent Nano Kit, v2 (500 cycles) 600,00 500Mbp /1 MLN
MiSeq Reagent Micro Kit, v2 (300 cycles) 700,00 1,2Gbp /4 MLN
NextSeq® 500/550 High Output Kit v2.5 (300 cycles) 4600,00 120Gbp /400 MLN
NextSeq® 500/550 Mid Output Kit v2.5 (300 cycles) 2100,00 39Gbp /130 MLN
NextSeq® 500/550 Mid Output Kit v2.5 (150 cycles) 1600,00 19Gbp /130 MLN
NextSeq® 500/550 High Output Kit v2.5 (150 cycles) 2900,00 60Gbp /400 MLN
NextSeq® 500/550 High Output Kit v2.5 (75 cycles) 1750,00 30Gbp /400 MLN

 

Bioinformatics

 

Type rate per sample
A B C
Amplicons taxonomic annotation (upstream: 16S, 18S, ITS) 18€ 19€ 20€
Amplicons taxonomic annotation and comparative analysis (downstream: 16S, 18S, ITS) 32€ 33€ 36€
Whole microbial genome mapping or small eukaryotes (< 10Mb) 46€ 47€ 51€
Metagenomes/Metatranscriptomes assembly and annotation 103€ 105€ 114€
Metagenomes/Metatranscriptomes assembly, annotation and differential analysis 143€ 145€ 158€
Metagenomes/Metatranscriptomes mapping and differential analysis 94€ 96€ 104€
Metagenomas/metatranscriptomas mapping 73€ 74€ 81€
Microbial genome comparative analysis, pangenome (<10Mb) 114€ 116€ 127€

 

A: FISABIO / Public Organizations of the Valencian Community
B: National and European Public Organizations
C: Private and International Organizations

 

Pending of approval: PACBIO SEQUEL II

 

Type rate per sample
A B C
Eukaryotic genomes HiFi (1 to 3 SMRT cells) 2594€ 2637€ 2875€
16S amplicones (96 samples per SMRT cell) 39€ 40€ 42€
Metagenomes (4 samples per SMRT cell) 983€ 999€ 1088€
GMicrobial genomes (min 40, max 48 samples) 203€ 206€ 225€