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FISABIO-Public Health Sequencing and Bioinformatics Service

Introduction

Over the last 10 years, DNA sequencing has experienced an exponential growth due to the emergence and development of mass sequencing techniques (NGS, Next Generation Sequencing). Currently, these initials encompass both the second generation sequencing platforms as well as the new third generation platforms. The implementation and development of these techniques open up new possibilities for analysis in multiple subdisciplines related to Biological and Health Sciences.

Some of the fields that are experiencing new advances thanks to mass sequencing are:

  • Clinical Microbiology, Public Health and Epidemiology.
  • Study of the microbial communities of the human body and their relationship with health and disease.
  • Microbiological safety in Public Health and in the agri-food industry.
  • Pest control.
  • Human genetics.

In order for these advances to take place, it is essential to have adequate sequencing platforms as well as professional personnel experienced in this type of techniques.

In this context and with the idea of promoting biomedical research, the Sequencing and Bioinformatics Service of FISABIO-Public Health was born.

The Service has independent laboratories and all the necessary equipment for the process of biological samples, purification and modification of nucleic acids, as well as for the construction of libraries and sequencing. The topographically separated laboratories of the service, also avoids contamination (both during the pre and post amplification of nucleic acids).

Also, the service is fully equipped with high performance calculation servers and backup units for the processing of bioinformatics and analyses of the data resulting from the sequencing runs.

Sequencing Infrastructure

Second generation

  • Illumina MiSeq: maximum capacity 2x300bp, total output up to 15 Gb.
  • Illumina NextSeq500: maximum capacity 2x150bp, total output up to 120 Gb.

Third generation

  • Oxford Nanopore MinIon: Long sequences, maximum capacity up to 100Kb, total output up to 30 Gb.
  • PacBio Sequel II (debuting on December 2019).